Quantification of evolved DNA-editing enzymes at scale with DEQSeq

We introduce DEQSeq, a nanopore sequencing approach that rationalizes the selection of favorable genome editing enzymes from directed molecular evolution experiments. With the ability to capture full-length sequences, editing efficiencies, and specificities from thousands of evolved enzymes simultan...

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Detalles Bibliográficos
Autores principales: Schmitt, Lukas Theo, Schneider, Aksana, Posorski, Jonas, Lansing, Felix, Jelicic, Milica, Jain, Manavi, Sayed, Shady, Buchholz, Frank, Sürün, Duran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10626641/
https://www.ncbi.nlm.nih.gov/pubmed/37932818
http://dx.doi.org/10.1186/s13059-023-03097-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10626641/
https://www.ncbi.nlm.nih.gov/pubmed/37932818
http://dx.doi.org/10.1186/s13059-023-03097-3

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