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Rapid and sensitive detection of Pseudomonas aeruginosa by isothermal amplification combined with Cas12a-mediated detection

CRISPR based technologies have been used for fast and sensitive detection of pathogens. To test the possibility of CRISPR based detection strategy in Pseudomonas aeruginosa infections, a combined method of recombinase polymerase amplification followed by Cas12a-mediated detection via fluorescence re...

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Detalles Bibliográficos
Autores principales: Huang, Siyi, Wang, Xianfeng, Chen, Xinchong, Liu, Xiaoyu, Xu, Qiuqing, Zhang, Lijun, Huang, Guangtao, Wu, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10628258/
https://www.ncbi.nlm.nih.gov/pubmed/37932335
http://dx.doi.org/10.1038/s41598-023-45766-0