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Fractionation of a sex-inducing substance from flatworms using open-column chromatography and reverse-phase high-performance liquid chromatography

A substance that sexualizes planarians, an ancestral group of parasitic flatworms, is widely present in planarians and parasitic flatworms. Here, we present a protocol for extracting and purifying the active fraction with sex-inducing activity. We describe steps for homogenization of flatworms, samp...

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Detalles Bibliográficos
Autores principales: Sekii, Kiyono, Watanabe, Taro, Ito, Riku, Yoshikawa, Akitoshi, Ichikawa-Seki, Madoka, Sakamoto, Kimitoshi, Kobayashi, Kazuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10628896/
http://dx.doi.org/10.1016/j.xpro.2023.102625