Melatonin inhibits Japanese encephalitis virus replication and neurotoxicity via calcineurin-autophagy pathways

BACKGROUND: Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus that has no specific treatment except for supportive medical care. JEV is a neurotropic virus that affects the nervous system and triggers inflammation in the brain. METHODS: Melatonin is used as a sleep-inducing agent in n...

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Autores principales: Moon, Ji-Hong, Hong, Jeong-Min, Seol, Jae-Won, Park, Byung-Yong, Eo, Seong Kug, Park, Sang-Youel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10629071/
https://www.ncbi.nlm.nih.gov/pubmed/37932682
http://dx.doi.org/10.1186/s12868-023-00832-1
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author Moon, Ji-Hong
Hong, Jeong-Min
Seol, Jae-Won
Park, Byung-Yong
Eo, Seong Kug
Park, Sang-Youel
author_facet Moon, Ji-Hong
Hong, Jeong-Min
Seol, Jae-Won
Park, Byung-Yong
Eo, Seong Kug
Park, Sang-Youel
author_sort Moon, Ji-Hong
collection PubMed
description BACKGROUND: Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus that has no specific treatment except for supportive medical care. JEV is a neurotropic virus that affects the nervous system and triggers inflammation in the brain. METHODS: Melatonin is used as a sleep-inducing agent in neurophysiology and may serve as a protective agent against neurological and neurodegenerative diseases. Herein, we investigated the effects of melatonin and the critical roles of the serine/threonine protein phosphatase calcineurin during JEV infection in SK-N-SH neuroblastoma cells. RESULTS: Melatonin treatment decreased JEV replication and JEV-mediated neurotoxicity. Calcineurin activity was increased by JEV infection and inhibited by melatonin treatment. Through calcineurin regulation, melatonin decreased the JEV-mediated neuroinflammatory response and attenuated JEV-induced autophagy. CONCLUSIONS: Calcineurin inactivation has a protective effect in JEV-infected neuronal cells, and melatonin is a novel resource for the development of anti-JEV agents. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12868-023-00832-1.
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spelling pubmed-106290712023-11-08 Melatonin inhibits Japanese encephalitis virus replication and neurotoxicity via calcineurin-autophagy pathways Moon, Ji-Hong Hong, Jeong-Min Seol, Jae-Won Park, Byung-Yong Eo, Seong Kug Park, Sang-Youel BMC Neurosci Research BACKGROUND: Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus that has no specific treatment except for supportive medical care. JEV is a neurotropic virus that affects the nervous system and triggers inflammation in the brain. METHODS: Melatonin is used as a sleep-inducing agent in neurophysiology and may serve as a protective agent against neurological and neurodegenerative diseases. Herein, we investigated the effects of melatonin and the critical roles of the serine/threonine protein phosphatase calcineurin during JEV infection in SK-N-SH neuroblastoma cells. RESULTS: Melatonin treatment decreased JEV replication and JEV-mediated neurotoxicity. Calcineurin activity was increased by JEV infection and inhibited by melatonin treatment. Through calcineurin regulation, melatonin decreased the JEV-mediated neuroinflammatory response and attenuated JEV-induced autophagy. CONCLUSIONS: Calcineurin inactivation has a protective effect in JEV-infected neuronal cells, and melatonin is a novel resource for the development of anti-JEV agents. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12868-023-00832-1. BioMed Central 2023-11-06 /pmc/articles/PMC10629071/ /pubmed/37932682 http://dx.doi.org/10.1186/s12868-023-00832-1 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Moon, Ji-Hong
Hong, Jeong-Min
Seol, Jae-Won
Park, Byung-Yong
Eo, Seong Kug
Park, Sang-Youel
Melatonin inhibits Japanese encephalitis virus replication and neurotoxicity via calcineurin-autophagy pathways
title Melatonin inhibits Japanese encephalitis virus replication and neurotoxicity via calcineurin-autophagy pathways
title_full Melatonin inhibits Japanese encephalitis virus replication and neurotoxicity via calcineurin-autophagy pathways
title_fullStr Melatonin inhibits Japanese encephalitis virus replication and neurotoxicity via calcineurin-autophagy pathways
title_full_unstemmed Melatonin inhibits Japanese encephalitis virus replication and neurotoxicity via calcineurin-autophagy pathways
title_short Melatonin inhibits Japanese encephalitis virus replication and neurotoxicity via calcineurin-autophagy pathways
title_sort melatonin inhibits japanese encephalitis virus replication and neurotoxicity via calcineurin-autophagy pathways
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10629071/
https://www.ncbi.nlm.nih.gov/pubmed/37932682
http://dx.doi.org/10.1186/s12868-023-00832-1
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