Preparation of site-specifically fluorophore-labeled polyubiquitin chains for FRET studies of Cdc48 substrate processing

A critical step in the removal of polyubiquitinated proteins from macromolecular complexes and membranes for subsequent proteasomal degradation is the unfolding of an ubiquitin moiety by the cofactor Ufd1/Npl4 (UN) and its insertion into the Cdc48 ATPase for mechanical translocation. Here, we presen...

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Detalles Bibliográficos
Autores principales: Williams, Cameron, Dong, Ken C., Arkinson, Connor, Martin, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10630674/
https://www.ncbi.nlm.nih.gov/pubmed/37889757
http://dx.doi.org/10.1016/j.xpro.2023.102659

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10630674/
https://www.ncbi.nlm.nih.gov/pubmed/37889757
http://dx.doi.org/10.1016/j.xpro.2023.102659

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