Protocol for establishing a protein interactome based on close physical proximity to a target protein within live budding yeast

Here, we present a protocol for establishing a protein interactome based on close physical proximity to a target protein within live yeast cells. We describe steps for capturing both transient and stable binders by integrating a non-natural amino acid. We detail procedures for employing a site-direc...

Descripción completa

Detalles Bibliográficos
Autores principales: Kolhe, Janhavi A., Babu, Neethu L., Freeman, Brian C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10630676/
https://www.ncbi.nlm.nih.gov/pubmed/37883222
http://dx.doi.org/10.1016/j.xpro.2023.102663

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10630676/
https://www.ncbi.nlm.nih.gov/pubmed/37883222
http://dx.doi.org/10.1016/j.xpro.2023.102663

Ejemplares similares