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Protocol for establishing a protein interactome based on close physical proximity to a target protein within live budding yeast
Here, we present a protocol for establishing a protein interactome based on close physical proximity to a target protein within live yeast cells. We describe steps for capturing both transient and stable binders by integrating a non-natural amino acid. We detail procedures for employing a site-direc...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10630676/ https://www.ncbi.nlm.nih.gov/pubmed/37883222 http://dx.doi.org/10.1016/j.xpro.2023.102663 |