Protocol for simultaneous analysis of peripheral and intratumoral lymphocyte function by flow cytometry

Here, we present a protocol for the simultaneous analysis of peripheral and intratumoral lymphocyte function by flow cytometry. Using tumor and blood samples from patients with colorectal cancer, we describe steps for tumor digestion, pre-labeling of the tumor-infiltrating leukocytes (TILs), and act...

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Detalles Bibliográficos
Autores principales: Daumoine, Susy, Hampe, Léa, Limagne, Emeric, Ghiringhelli, François, Thibaudin, Marion
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10630783/
https://www.ncbi.nlm.nih.gov/pubmed/37910510
http://dx.doi.org/10.1016/j.xpro.2023.102658

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10630783/
https://www.ncbi.nlm.nih.gov/pubmed/37910510
http://dx.doi.org/10.1016/j.xpro.2023.102658

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