Protocol for simultaneous analysis of peripheral and intratumoral lymphocyte function by flow cytometry

Here, we present a protocol for the simultaneous analysis of peripheral and intratumoral lymphocyte function by flow cytometry. Using tumor and blood samples from patients with colorectal cancer, we describe steps for tumor digestion, pre-labeling of the tumor-infiltrating leukocytes (TILs), and act...

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Detalles Bibliográficos
Autores principales: Daumoine, Susy, Hampe, Léa, Limagne, Emeric, Ghiringhelli, François, Thibaudin, Marion
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10630783/
https://www.ncbi.nlm.nih.gov/pubmed/37910510
http://dx.doi.org/10.1016/j.xpro.2023.102658
Descripción
Sumario:Here, we present a protocol for the simultaneous analysis of peripheral and intratumoral lymphocyte function by flow cytometry. Using tumor and blood samples from patients with colorectal cancer, we describe steps for tumor digestion, pre-labeling of the tumor-infiltrating leukocytes (TILs), and activation and labeling of the total cells (TILs and whole blood cells). For complete details on the use and execution of this protocol, please refer to Thibaudin et al.(1)

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