Protocol for simultaneous analysis of peripheral and intratumoral lymphocyte function by flow cytometry

Here, we present a protocol for the simultaneous analysis of peripheral and intratumoral lymphocyte function by flow cytometry. Using tumor and blood samples from patients with colorectal cancer, we describe steps for tumor digestion, pre-labeling of the tumor-infiltrating leukocytes (TILs), and act...

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Autores principales: Daumoine, Susy, Hampe, Léa, Limagne, Emeric, Ghiringhelli, François, Thibaudin, Marion
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10630783/
https://www.ncbi.nlm.nih.gov/pubmed/37910510
http://dx.doi.org/10.1016/j.xpro.2023.102658
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author Daumoine, Susy
Hampe, Léa
Limagne, Emeric
Ghiringhelli, François
Thibaudin, Marion
author_facet Daumoine, Susy
Hampe, Léa
Limagne, Emeric
Ghiringhelli, François
Thibaudin, Marion
author_sort Daumoine, Susy
collection PubMed
description Here, we present a protocol for the simultaneous analysis of peripheral and intratumoral lymphocyte function by flow cytometry. Using tumor and blood samples from patients with colorectal cancer, we describe steps for tumor digestion, pre-labeling of the tumor-infiltrating leukocytes (TILs), and activation and labeling of the total cells (TILs and whole blood cells). For complete details on the use and execution of this protocol, please refer to Thibaudin et al.(1)
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spelling pubmed-106307832023-10-31 Protocol for simultaneous analysis of peripheral and intratumoral lymphocyte function by flow cytometry Daumoine, Susy Hampe, Léa Limagne, Emeric Ghiringhelli, François Thibaudin, Marion STAR Protoc Protocol Here, we present a protocol for the simultaneous analysis of peripheral and intratumoral lymphocyte function by flow cytometry. Using tumor and blood samples from patients with colorectal cancer, we describe steps for tumor digestion, pre-labeling of the tumor-infiltrating leukocytes (TILs), and activation and labeling of the total cells (TILs and whole blood cells). For complete details on the use and execution of this protocol, please refer to Thibaudin et al.(1) Elsevier 2023-10-31 /pmc/articles/PMC10630783/ /pubmed/37910510 http://dx.doi.org/10.1016/j.xpro.2023.102658 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Daumoine, Susy
Hampe, Léa
Limagne, Emeric
Ghiringhelli, François
Thibaudin, Marion
Protocol for simultaneous analysis of peripheral and intratumoral lymphocyte function by flow cytometry
title Protocol for simultaneous analysis of peripheral and intratumoral lymphocyte function by flow cytometry
title_full Protocol for simultaneous analysis of peripheral and intratumoral lymphocyte function by flow cytometry
title_fullStr Protocol for simultaneous analysis of peripheral and intratumoral lymphocyte function by flow cytometry
title_full_unstemmed Protocol for simultaneous analysis of peripheral and intratumoral lymphocyte function by flow cytometry
title_short Protocol for simultaneous analysis of peripheral and intratumoral lymphocyte function by flow cytometry
title_sort protocol for simultaneous analysis of peripheral and intratumoral lymphocyte function by flow cytometry
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10630783/
https://www.ncbi.nlm.nih.gov/pubmed/37910510
http://dx.doi.org/10.1016/j.xpro.2023.102658
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