Impact of library input on the hit discovery rate in DNA-encoded chemical library selections

DNA-encoded chemical libraries (DELs) are powerful drug discovery tools, enabling the parallel screening of millions of DNA-barcoded compounds. We investigated how the DEL input affects the hit discovery rate in DEL screenings. Evaluation of selection fingerprints revealed that the use of approximat...

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Detalles Bibliográficos
Autores principales: Puglioli, Sara, Oehler, Sebastian, Prati, Luca, Scheuermann, Jörg, Bassi, Gabriele, Cazzamalli, Samuele, Neri, Dario, Favalli, Nicholas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2023
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10631129/
https://www.ncbi.nlm.nih.gov/pubmed/37969600
http://dx.doi.org/10.1039/d3sc03688j
Descripción
Sumario:DNA-encoded chemical libraries (DELs) are powerful drug discovery tools, enabling the parallel screening of millions of DNA-barcoded compounds. We investigated how the DEL input affects the hit discovery rate in DEL screenings. Evaluation of selection fingerprints revealed that the use of approximately 10(5) copies of each library member is required for the confident identification of nanomolar hits, using generally applicable methodologies.

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