Biosynthesis and Genetic Encoding of Non-hydrolyzable Phosphoserine into Recombinant Proteins in Escherichia coli

While site-specific translational encoding of phosphoserine (pSer) into proteins in Escherichia coli via genetic code expansion (GCE) technologies has transformed our ability to study phospho-protein structure and function, recombinant phospho-proteins can be dephosphorylated during expression/purif...

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Detalles Bibliográficos
Autores principales: Zhu, Phillip, Mehl, Ryan A., Cooley, Richard B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bio-Protocol 2023
Materias:
Methods Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10632156/
https://www.ncbi.nlm.nih.gov/pubmed/37969748
http://dx.doi.org/10.21769/BioProtoc.4861

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10632156/
https://www.ncbi.nlm.nih.gov/pubmed/37969748
http://dx.doi.org/10.21769/BioProtoc.4861

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