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Biosynthesis and Genetic Encoding of Non-hydrolyzable Phosphoserine into Recombinant Proteins in Escherichia coli

While site-specific translational encoding of phosphoserine (pSer) into proteins in Escherichia coli via genetic code expansion (GCE) technologies has transformed our ability to study phospho-protein structure and function, recombinant phospho-proteins can be dephosphorylated during expression/purif...

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Detalles Bibliográficos
Autores principales:	Zhu, Phillip, Mehl, Ryan A., Cooley, Richard B.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Bio-Protocol 2023
Materias:	Methods Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10632156/ https://www.ncbi.nlm.nih.gov/pubmed/37969748 http://dx.doi.org/10.21769/BioProtoc.4861

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