Optimization of high-throughput 16S rRNA gene amplicon sequencing: an assessment of PCR pooling, mastermix use and contamination

16S rRNA gene sequencing is widely used to characterize human and environmental microbiomes. Sequencing at scale facilitates better powered studies but is limited by cost and time. We identified two areas in our 16S rRNA gene library preparation protocol where modifications could provide efficiency...

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Detalles Bibliográficos
Autores principales: Aggarwal, Dinesh, Rajan, Diana, Bellis, Katherine L., Betteridge, Emma, Brennan, Joe, de Sousa, Catarina, Parkhill, Julian, Peacock, Sharon J., de Goffau, Marcus C., Wagner, Josef, Harrison, Ewan M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2023
Materias:
Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10634443/
https://www.ncbi.nlm.nih.gov/pubmed/37843887
http://dx.doi.org/10.1099/mgen.0.001115

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10634443/
https://www.ncbi.nlm.nih.gov/pubmed/37843887
http://dx.doi.org/10.1099/mgen.0.001115

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