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Flow cytometry-based quantification of genome editing efficiency in human cell lines using the L1CAM gene
CRISPR/Cas9 is a powerful genome editing system that has remarkably facilitated gene knockout and targeted knock-in. To accelerate the practical use of CRISPR/Cas9, however, it remains crucial to improve the efficiency, precision, and specificity of genome editing, particularly targeted knock-in, ac...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10635454/ https://www.ncbi.nlm.nih.gov/pubmed/37943774 http://dx.doi.org/10.1371/journal.pone.0294146 |