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Microfluidics-free single-cell genomics with templated emulsification

Current single-cell RNA-sequencing approaches have limitations that stem from the microfluidic devices or fluid handling steps required for sample processing. We develop a method that does not require specialized microfluidic devices, expertise or hardware. Our approach is based on particle-template...

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Detalles Bibliográficos
Autores principales: Clark, Iain C., Fontanez, Kristina M., Meltzer, Robert H., Xue, Yi, Hayford, Corey, May-Zhang, Aaron, D’Amato, Chris, Osman, Ahmad, Zhang, Jesse Q., Hettige, Pabodha, Ishibashi, Jacob S. A., Delley, Cyrille L., Weisgerber, Daniel W., Replogle, Joseph M., Jost, Marco, Phong, Kiet T., Kennedy, Vanessa E., Peretz, Cheryl A. C., Kim, Esther A., Song, Siyou, Karlon, William, Weissman, Jonathan S., Smith, Catherine C., Gartner, Zev J., Abate, Adam R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10635830/
https://www.ncbi.nlm.nih.gov/pubmed/36879006
http://dx.doi.org/10.1038/s41587-023-01685-z