Analysis of microbial composition and sharing in low-biomass human milk samples: a comparison of DNA isolation and sequencing techniques

Human milk microbiome studies are currently hindered by low milk bacterial/human cell ratios and often rely on 16S rRNA gene sequencing, which limits downstream analyses. Here, we aimed to find a method to study milk bacteria and assess bacterial sharing between maternal and infant microbiota. We te...

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Detalles Bibliográficos
Autores principales: Spreckels, Johanne E., Fernández-Pato, Asier, Kruk, Marloes, Kurilshikov, Alexander, Garmaeva, Sanzhima, Sinha, Trishla, Ghosh, Hiren, Harmsen, Hermie, Fu, Jingyuan, Gacesa, Ranko, Zhernakova, Alexandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10636111/
https://www.ncbi.nlm.nih.gov/pubmed/37945978
http://dx.doi.org/10.1038/s43705-023-00325-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10636111/
https://www.ncbi.nlm.nih.gov/pubmed/37945978
http://dx.doi.org/10.1038/s43705-023-00325-6

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