Ablation of OCT4 function in cattle embryos by double electroporation of CRISPR-Cas for DNA and RNA targeting (CRISPR-DART)

CRISPR-Cas ribonucleoproteins (RNPs) are important tools for gene editing in preimplantation embryos. However, the inefficient production of biallelic deletions in cattle zygotes has hindered mechanistic studies of gene function. In addition, the presence of maternal RNAs that support embryo develop...

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Detalles Bibliográficos
Autores principales: Nix, Jada L, Schettini, Gustavo P, Speckhart, Savannah L, Ealy, Alan D, Biase, Fernando H
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Biological, Health, and Medical Sciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10637268/
https://www.ncbi.nlm.nih.gov/pubmed/37954164
http://dx.doi.org/10.1093/pnasnexus/pgad343

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10637268/
https://www.ncbi.nlm.nih.gov/pubmed/37954164
http://dx.doi.org/10.1093/pnasnexus/pgad343

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