Cargando…

Ablation of OCT4 function in cattle embryos by double electroporation of CRISPR-Cas for DNA and RNA targeting (CRISPR-DART)

CRISPR-Cas ribonucleoproteins (RNPs) are important tools for gene editing in preimplantation embryos. However, the inefficient production of biallelic deletions in cattle zygotes has hindered mechanistic studies of gene function. In addition, the presence of maternal RNAs that support embryo develop...

Descripción completa

Detalles Bibliográficos
Autores principales:	Nix, Jada L, Schettini, Gustavo P, Speckhart, Savannah L, Ealy, Alan D, Biase, Fernando H
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2023
Materias:	Biological, Health, and Medical Sciences
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10637268/ https://www.ncbi.nlm.nih.gov/pubmed/37954164 http://dx.doi.org/10.1093/pnasnexus/pgad343

Ejemplares similares

Sexing of cattle embryos using RNA-sequencing data or polymerase chain reaction based on a complete sequence of cattle chromosome Y
por: Nix, Jada Lindsay, et al.
Publicado: (2023)

Cytokines That Serve as Embryokines in Cattle
por: Ealy, Alan D., et al.
Publicado: (2021)

An updated protocol for in vitro bovine embryo production
por: Speckhart, Savannah L., et al.
Publicado: (2022)

Generation of double knockout cattle via CRISPR-Cas9 ribonucleoprotein (RNP) electroporation
por: Gim, Gyeong-Min, et al.
Publicado: (2023)

Developmental Hurdles That Can Compromise Pregnancy during the First Month of Gestation in Cattle
por: Speckhart, Savannah L., et al.
Publicado: (2023)

Tight gene co-expression in BCB positive cattle oocytes and their surrounding cumulus cells
por: Walker, Bailey N., et al.
Publicado: (2022)

Comparison of the effects of introducing the CRISPR/Cas9 system by microinjection and electroporation into porcine embryos at different stages
por: Le, Quynh Anh, et al.
Publicado: (2021)

TIE: A Method to Electroporate Long DNA Templates into Preimplantation Embryos for CRISPR-Cas9 Gene Editing
por: Bagheri, Hooman, et al.
Publicado: (2018)

Efficient DNA knock-in using AAV-mediated delivery with 2-cell embryo CRISPR-Cas9 electroporation
por: Davis, Daniel J., et al.
Publicado: (2023)

Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease
por: Qin, Wenning, et al.
Publicado: (2015)

Effects of the timing of electroporation during in vitro maturation on triple gene editing in porcine embryos using CRISPR/Cas9 system
por: Namula, Zhao, et al.
Publicado: (2022)

CRISPR/Cas9-mediated gene knockout in the mouse brain using in utero electroporation
por: Shinmyo, Yohei, et al.
Publicado: (2016)

Genome editing of Ralstonia eutropha using an electroporation-based CRISPR-Cas9 technique
por: Xiong, Bin, et al.
Publicado: (2018)

An optimized electroporation approach for efficient CRISPR/Cas9 genome editing in murine zygotes
por: Tröder, Simon E., et al.
Publicado: (2018)

Validation of gene editing efficiency with CRISPR-Cas9 system directly in rat zygotes using electroporation mediated delivery and embryo culture
por: Challa, Anil K., et al.
Publicado: (2021)

CRISPR/Cas9 technology: applications in oocytes and early embryos
por: Zhang, Yi-ran, et al.
Publicado: (2023)

A ‘suicide’ CRISPR-Cas9 system to promote gene deletion and restoration by electroporation in Cryptococcus neoformans
por: Wang, Yu, et al.
Publicado: (2016)

Rapid generation of conditional knockout mice using the CRISPR-Cas9 system and electroporation for neuroscience research
por: Nishizono, Hirofumi, et al.
Publicado: (2021)

Pigs with an INS point mutation derived from zygotes electroporated with CRISPR/Cas9 and ssODN
por: Tanihara, Fuminori, et al.
Publicado: (2023)

CRISPR/Cas-based customization of pooled CRISPR libraries
por: Kweon, Jiyeon, et al.
Publicado: (2018)

Anti‐CRISPRs: The natural inhibitors for CRISPR‐Cas systems
por: Zhang, Fei, et al.
Publicado: (2019)

In vivo Editing of the Human Mutant Rhodopsin Gene by Electroporation of Plasmid-based CRISPR/Cas9 in the Mouse Retina
por: Latella, Maria Carmela, et al.
Publicado: (2016)

Efficient generation of GGTA1-deficient pigs by electroporation of the CRISPR/Cas9 system into in vitro-fertilized zygotes
por: Tanihara, Fuminori, et al.
Publicado: (2020)

Modification of a Chlamydomonas reinhardtii CRISPR/Cas9 transformation protocol for use with widely available electroporation equipment
por: Park, Rudolph V., et al.
Publicado: (2020)

Optimization of sand fly embryo microinjection for gene editing by CRISPR/Cas9
por: Martin-Martin, Ines, et al.
Publicado: (2018)

Optogenetic regulation of embryo implantation in mice using photoactivatable CRISPR-Cas9
por: Takao, Tomoka, et al.
Publicado: (2020)

Lipofection-Mediated Introduction of CRISPR/Cas9 System into Porcine Oocytes and Embryos
por: Hirata, Maki, et al.
Publicado: (2021)

Genome editing of CCR5 by CRISPR-Cas9 in Mauritian cynomolgus macaque embryos
por: Schmidt, Jenna Kropp, et al.
Publicado: (2020)

Diagnostics of Infections Produced by the Plant Viruses TMV, TEV, and PVX with CRISPR-Cas12 and CRISPR-Cas13
por: Marqués, María-Carmen, et al.
Publicado: (2022)

Cas6 specificity and CRISPR RNA loading in a complex CRISPR-Cas system
por: Sokolowski, Richard D., et al.
Publicado: (2014)

Anti-CRISPR Phages Cooperate to Overcome CRISPR-Cas Immunity
por: Landsberger, Mariann, et al.
Publicado: (2018)

Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing
por: Hashimoto, Masakazu, et al.
Publicado: (2015)

Generation of gene-edited rats by delivery of CRISPR/Cas9 protein and donor DNA into intact zygotes using electroporation
por: Remy, Séverine, et al.
Publicado: (2017)

Efficient homology-directed gene editing by CRISPR/Cas9 in human stem and primary cells using tube electroporation
por: Xu, Xiaoyun, et al.
Publicado: (2018)

CRISPR-Cas adaptation in Escherichia coli
por: Mitić, Damjan, et al.
Publicado: (2023)

Rationally Designed Anti-CRISPR Nucleic Acid Inhibitors of CRISPR-Cas9
por: Barkau, Christopher L., et al.
Publicado: (2019)

Special focus CRISPR-Cas
por: Marchfelder, Anita
Publicado: (2013)

What is CRISPR/Cas9?
por: Redman, Melody, et al.
Publicado: (2016)

CRISPR-Cas in Streptococcus pyogenes
por: Le Rhun, Anaïs, et al.
Publicado: (2019)

Advances in CRISPR/Cas9
por: Zhu, Youmin
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_em26j6uhpsh9lbsu41h3vudibr