Mechanistic aspects of IPTG (isopropylthio-β-galactoside) transport across the cytoplasmic membrane of Escherichia coli—a rate limiting step in the induction of recombinant protein expression

 : Coupling transcription of a cloned gene to the lac operon with induction by isopropylthio-β-galactoside (IPTG) has been a favoured approach for recombinant protein expression using Escherichia coli as a heterologous host for more than six decades. Despite a wealth of experimental data gleaned ove...

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Detalles Bibliográficos
Autores principales: Simas, Rodrigo G, Pessoa Junior, Adalberto, Long, Paul F
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Fermentation, Cell Culture and Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10639102/
https://www.ncbi.nlm.nih.gov/pubmed/37849239
http://dx.doi.org/10.1093/jimb/kuad034

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10639102/
https://www.ncbi.nlm.nih.gov/pubmed/37849239
http://dx.doi.org/10.1093/jimb/kuad034

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