Protocol for editing fibroblasts with in vitro transcribed Cas9 mRNA and profile off-target editing by optimized GUIDE-seq

CRISPR-Cas9 gene editing is an efficient technique to modify specific sites/regions of DNA. Delivery of the Cas9 by mRNA is particularly promising in pre-clinical genome editing applications for its transient, nonintegrating feature. However, the off-target of Cas9-gRNA still remains a concern and n...

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Detalles Bibliográficos
Autores principales: Li, Zhuokun, Reint, Ganna, Haapaniemi, Emma Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10641304/
https://www.ncbi.nlm.nih.gov/pubmed/37889758
http://dx.doi.org/10.1016/j.xpro.2023.102662

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10641304/
https://www.ncbi.nlm.nih.gov/pubmed/37889758
http://dx.doi.org/10.1016/j.xpro.2023.102662

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