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Protocol to quantify glycan-mediated binding between viral particles and human peripheral blood mononuclear cells using nanoluciferase

Prior to receptor engagement, a specific, non-electrostatic glycan-glycan interaction between viral particles and host cells may lengthen the dwell time of the virus at the cellular surface, thereby facilitating subsequent virus entry. Here, we present a protocol for quantifying the level of glycan-...

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Detalles Bibliográficos
Autores principales: Spillings, Belinda L., Mak, Johnson
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10641309/
https://www.ncbi.nlm.nih.gov/pubmed/37889756
http://dx.doi.org/10.1016/j.xpro.2023.102672