Shifted PAMs generate DNA overhangs and enhance SpCas9 post-catalytic complex dissociation

Using Sanger sequencing and high-throughput genome sequencing of DNA cleavage reactions, we find that the Streptococcus pyogenes SpCas9 complex responds to internal mechanical strain by robustly generating a distribution of overhanging, rather than blunt, DNA ends. Internal mechanical strain is gene...

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Detalles Bibliográficos
Autores principales: Wang, Jinglong, Le Gall, Julien, Frock, Richard L., Strick, Terence R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10643121/
https://www.ncbi.nlm.nih.gov/pubmed/37828409
http://dx.doi.org/10.1038/s41594-023-01104-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10643121/
https://www.ncbi.nlm.nih.gov/pubmed/37828409
http://dx.doi.org/10.1038/s41594-023-01104-6

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