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Shifted PAMs generate DNA overhangs and enhance SpCas9 post-catalytic complex dissociation
Using Sanger sequencing and high-throughput genome sequencing of DNA cleavage reactions, we find that the Streptococcus pyogenes SpCas9 complex responds to internal mechanical strain by robustly generating a distribution of overhanging, rather than blunt, DNA ends. Internal mechanical strain is gene...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group US
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10643121/ https://www.ncbi.nlm.nih.gov/pubmed/37828409 http://dx.doi.org/10.1038/s41594-023-01104-6 |