Cargando…

CHEX-seq detects single-cell genomic single-stranded DNA with catalytical potential

Genomic DNA (gDNA) undergoes structural interconversion between single- and double-stranded states during transcription, DNA repair and replication, which is critical for cellular homeostasis. We describe “CHEX-seq” which identifies the single-stranded DNA (ssDNA) in situ in individual cells. CHEX-s...

Descripción completa

Detalles Bibliográficos
Autores principales:	Lu, Youtao, Lee, Jaehee, Li, Jifen, Allu, Srinivasa Rao, Wang, Jinhui, Kim, HyunBum, Bullaughey, Kevin L., Fisher, Stephen A., Nordgren, C. Erik, Rosario, Jean G., Anderson, Stewart A., Ulyanova, Alexandra V., Brem, Steven, Chen, H. Isaac, Wolf, John A., Grady, M. Sean, Vinogradov, Sergei A., Kim, Junhyong, Eberwine, James
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10645931/ https://www.ncbi.nlm.nih.gov/pubmed/37963886 http://dx.doi.org/10.1038/s41467-023-43158-6

Ejemplares similares

Variation is function: Are single cell differences functionally important?: Testing the hypothesis that single cell variation is required for aggregate function
por: Dueck, Hannah, et al.
Publicado: (2015)

Comprehensive catalog of dendritically localized mRNA isoforms from sub-cellular sequencing of single mouse neurons
por: Middleton, Sarah A., et al.
Publicado: (2019)

Transcriptome In Vivo Analysis (TIVA) of spatially defined single cells in intact live mouse and human brain tissue
por: Lovatt, Ditte, et al.
Publicado: (2014)

Astrocyte ethanol exposure reveals persistent and defined calcium response subtypes and associated gene signatures
por: Kim, Hyun-Bum, et al.
Publicado: (2022)

Quantitative biology of single neurons
por: Eberwine, James, et al.
Publicado: (2012)

The successes and future prospects of the linear antisense RNA amplification methodology
por: Li, Jifen, et al.
Publicado: (2018)

The HtrA protease of Borrelia burgdorferi degrades outer membrane protein BmpD and chemotaxis phosphatase CheX
por: Coleman, James L, et al.
Publicado: (2013)

The Effect of Selective D- or N(α)-Methyl Arginine Substitution on the Activity of the Proline-Rich Antimicrobial Peptide, Chex1-Arg20
por: Li, Wenyi, et al.
Publicado: (2017)

XRCC1 is required for DNA single-strand break repair in human cells
por: Brem, Reto, et al.
Publicado: (2005)

Deep sequencing reveals cell-type-specific patterns of single-cell transcriptome variation
por: Dueck, Hannah, et al.
Publicado: (2015)

Avian Primordial Germ Cells Contribute to and Interact With the Extracellular Matrix During Early Migration
por: Huss, David J., et al.
Publicado: (2019)

Ribonuclease from Bacillus Acts as an Antiviral Agent against Negative- and Positive-Sense Single Stranded Human Respiratory RNA Viruses
por: Shah Mahmud, Raihan, et al.
Publicado: (2017)

Hypermutation in single-stranded DNA
por: Saini, Natalie, et al.
Publicado: (2020)

Transcriptome Analysis of Single Cells
por: Morris, Jacqueline, et al.
Publicado: (2011)

Robust Genome Editing with Short Single-Stranded and Long, Partially Single-Stranded DNA Donors in Caenorhabditis elegans
por: Dokshin, Gregoriy A., et al.
Publicado: (2018)

Divergence of RNA localization between rat and mouse neurons reveals the potential for rapid brain evolution
por: Francis, Chantal, et al.
Publicado: (2014)

Single-Stranded RNA Viruses
por: Lvov, Dimitry Konstantinovich, et al.
Publicado: (2015)

Single-Strand Annealing in Cancer
por: Blasiak, Janusz
Publicado: (2021)

Protected DNA strand displacement for enhanced single nucleotide discrimination in double-stranded DNA
por: Khodakov, Dmitriy A., et al.
Publicado: (2015)

Assessing characteristics of RNA amplification methods for single cell RNA sequencing
por: Dueck, Hannah R., et al.
Publicado: (2016)

Single Cell Transcriptomic Analysis Reveals Organ Specific Pericyte Markers and Identities
por: Baek, Seung-Han, et al.
Publicado: (2022)

Physical and Functional Interaction of Mitochondrial Single-Stranded DNA-Binding Protein and the Catalytic Subunit of DNA Polymerase Gamma
por: Ciesielski, Grzegorz L., et al.
Publicado: (2021)

Plasmid replication-associated single-strand-specific methyltransferases
por: Fomenkov, Alexey, et al.
Publicado: (2020)

AAV Recombineering with Single Strand Oligonucleotides
por: Hirsch, Matthew L., et al.
Publicado: (2009)

Interaction of PKR with single-stranded RNA
por: Mayo, Christopher B., et al.
Publicado: (2017)

Single-stranded circular DNA theranostics
por: Shen, Tingting, et al.
Publicado: (2022)

Fluorescent single-stranded DNA-binding protein from Plasmodium falciparum as a biosensor for single-stranded DNA
por: Chisty, Liisa T., et al.
Publicado: (2018)

Co-condensation of proteins with single- and double-stranded DNA
por: Renger, Roman, et al.
Publicado: (2022)

Single-strand mismatch and damage patterns revealed by single-molecule DNA sequencing
por: Liu, Mei Hong, et al.
Publicado: (2023)

Hypermutability of Damaged Single-Strand DNA Formed at Double-Strand Breaks and Uncapped Telomeres in Yeast Saccharomyces cerevisiae
por: Yang, Yong, et al.
Publicado: (2008)

Accounting for technical noise in differential expression analysis of single-cell RNA sequencing data
por: Jia, Cheng, et al.
Publicado: (2017)

DENT-seq for genome-wide strand-specific identification of DNA single-strand break sites with single-nucleotide resolution
por: Elacqua, Joshua J., et al.
Publicado: (2021)

Subcellular RNA Sequencing Reveals Broad Presence of Cytoplasmic Intron-Sequence Retaining Transcripts in Mouse and Rat Neurons
por: Khaladkar, Mugdha, et al.
Publicado: (2013)

Excess Single-Stranded DNA Inhibits Meiotic Double-Strand Break Repair
por: Johnson, Rebecca, et al.
Publicado: (2007)

IS200/IS605 family single-strand transposition: mechanism of IS608 strand transfer
por: He, Susu, et al.
Publicado: (2013)

Coordinated Binding of Single-Stranded and Double-Stranded DNA by UvsX Recombinase
por: Maher, Robyn L., et al.
Publicado: (2013)

Towards quantitative viromics for both double-stranded and single-stranded DNA viruses
por: Roux, Simon, et al.
Publicado: (2016)

Sequence-dependent quenching of fluorescein fluorescence on single-stranded and double-stranded DNA
por: Lietard, Jory, et al.
Publicado: (2022)

Strand asymmetry influences mismatch resolution during a single-strand annealing
por: Pokusaeva, Victoria O., et al.
Publicado: (2022)

Single-stranded heteroduplex intermediates in λ Red homologous recombination
por: Maresca, Marcello, et al.
Publicado: (2010)

Cannot write session to /tmp/vufind_sessions/sess_tne907uhsckbne5gq9dmm2t8c0