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In the skin lesions of patients with mycosis fungoides, the number of MRGPRX2-expressing cells is increased and correlates with mast cell numbers
BACKGROUND: Mycosis fungoides (MF) is an indolent T-cell lymphoma that mainly affects the skin and presents with itch in more than half of the patients. Recently, the expression of Mas-related G protein-coupled receptor X2 (MRGPRX2), a receptor of mast cell (MC) responsible for the IgE-independent n...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10646224/ https://www.ncbi.nlm.nih.gov/pubmed/38022672 http://dx.doi.org/10.3389/fimmu.2023.1197821 |
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author | Hu, Man Pyatilova, Polina Altrichter, Sabine Sheng, Caibin Liu, Nian Terhorst-Molawi, Dorothea Lohse, Katharina Ginter, Katharina Puhl, Viktoria Maurer, Marcus Metz, Martin Kolkhir, Pavel |
author_facet | Hu, Man Pyatilova, Polina Altrichter, Sabine Sheng, Caibin Liu, Nian Terhorst-Molawi, Dorothea Lohse, Katharina Ginter, Katharina Puhl, Viktoria Maurer, Marcus Metz, Martin Kolkhir, Pavel |
author_sort | Hu, Man |
collection | PubMed |
description | BACKGROUND: Mycosis fungoides (MF) is an indolent T-cell lymphoma that mainly affects the skin and presents with itch in more than half of the patients. Recently, the expression of Mas-related G protein-coupled receptor X2 (MRGPRX2), a receptor of mast cell (MC) responsible for the IgE-independent non-histaminergic itch, has been shown in lesional skin of patients with pruritic skin diseases, including chronic urticaria, prurigo, and mastocytosis. As of yet, limited knowledge exists regarding the MRGPRX2 expression in the skin of patients with MF. OBJECTIVES: To investigate the number of MRGPRX2-expressing (MRGPRX2+) cells in the skin of patients with MF and its correlation with clinical and laboratory characteristics of the disease. METHODS: MRGPRX2 was analyzed in lesional and non-lesional skin of MF patients and healthy skin tissues by immunohistochemistry. Co-localization of MRGPRX2 with the MC marker tryptase was assessed by immunofluorescence. Public single-cell RNAseq data was reanalyzed to identify the MRGPRX2 expression on the distinct cell types. RESULTS: In lesional skin of MF patients, MRGPRX2+ cell number was higher than in non-lesional skin and healthy control skin (mean:15.12 vs. 6.84 vs. 5.51 cells/mm(2), p=0.04), and correlated with MC numbers (r=0.73, p=0.02). MC was the primary cell type expressing MRGPRX2 in MF patients. The ratio of MRGPRX2+ MCs to MRGPRX2+ cells in lesional and non-lesional skin correlated with the severity of disease (r=0.71, p=0.02 and r=0.67, p=0.03, respectively). CONCLUSIONS: Our findings point to the role of MRGPRX2 and MC in the pathogenesis of MF that should be investigated in further studies. |
format | Online Article Text |
id | pubmed-10646224 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-106462242023-01-01 In the skin lesions of patients with mycosis fungoides, the number of MRGPRX2-expressing cells is increased and correlates with mast cell numbers Hu, Man Pyatilova, Polina Altrichter, Sabine Sheng, Caibin Liu, Nian Terhorst-Molawi, Dorothea Lohse, Katharina Ginter, Katharina Puhl, Viktoria Maurer, Marcus Metz, Martin Kolkhir, Pavel Front Immunol Immunology BACKGROUND: Mycosis fungoides (MF) is an indolent T-cell lymphoma that mainly affects the skin and presents with itch in more than half of the patients. Recently, the expression of Mas-related G protein-coupled receptor X2 (MRGPRX2), a receptor of mast cell (MC) responsible for the IgE-independent non-histaminergic itch, has been shown in lesional skin of patients with pruritic skin diseases, including chronic urticaria, prurigo, and mastocytosis. As of yet, limited knowledge exists regarding the MRGPRX2 expression in the skin of patients with MF. OBJECTIVES: To investigate the number of MRGPRX2-expressing (MRGPRX2+) cells in the skin of patients with MF and its correlation with clinical and laboratory characteristics of the disease. METHODS: MRGPRX2 was analyzed in lesional and non-lesional skin of MF patients and healthy skin tissues by immunohistochemistry. Co-localization of MRGPRX2 with the MC marker tryptase was assessed by immunofluorescence. Public single-cell RNAseq data was reanalyzed to identify the MRGPRX2 expression on the distinct cell types. RESULTS: In lesional skin of MF patients, MRGPRX2+ cell number was higher than in non-lesional skin and healthy control skin (mean:15.12 vs. 6.84 vs. 5.51 cells/mm(2), p=0.04), and correlated with MC numbers (r=0.73, p=0.02). MC was the primary cell type expressing MRGPRX2 in MF patients. The ratio of MRGPRX2+ MCs to MRGPRX2+ cells in lesional and non-lesional skin correlated with the severity of disease (r=0.71, p=0.02 and r=0.67, p=0.03, respectively). CONCLUSIONS: Our findings point to the role of MRGPRX2 and MC in the pathogenesis of MF that should be investigated in further studies. Frontiers Media S.A. 2023-10-30 /pmc/articles/PMC10646224/ /pubmed/38022672 http://dx.doi.org/10.3389/fimmu.2023.1197821 Text en Copyright © 2023 Hu, Pyatilova, Altrichter, Sheng, Liu, Terhorst-Molawi, Lohse, Ginter, Puhl, Maurer, Metz and Kolkhir https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Hu, Man Pyatilova, Polina Altrichter, Sabine Sheng, Caibin Liu, Nian Terhorst-Molawi, Dorothea Lohse, Katharina Ginter, Katharina Puhl, Viktoria Maurer, Marcus Metz, Martin Kolkhir, Pavel In the skin lesions of patients with mycosis fungoides, the number of MRGPRX2-expressing cells is increased and correlates with mast cell numbers |
title | In the skin lesions of patients with mycosis fungoides, the number of MRGPRX2-expressing cells is increased and correlates with mast cell numbers |
title_full | In the skin lesions of patients with mycosis fungoides, the number of MRGPRX2-expressing cells is increased and correlates with mast cell numbers |
title_fullStr | In the skin lesions of patients with mycosis fungoides, the number of MRGPRX2-expressing cells is increased and correlates with mast cell numbers |
title_full_unstemmed | In the skin lesions of patients with mycosis fungoides, the number of MRGPRX2-expressing cells is increased and correlates with mast cell numbers |
title_short | In the skin lesions of patients with mycosis fungoides, the number of MRGPRX2-expressing cells is increased and correlates with mast cell numbers |
title_sort | in the skin lesions of patients with mycosis fungoides, the number of mrgprx2-expressing cells is increased and correlates with mast cell numbers |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10646224/ https://www.ncbi.nlm.nih.gov/pubmed/38022672 http://dx.doi.org/10.3389/fimmu.2023.1197821 |
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