Comparison of Multiple Strategies for Precision Transgene Knock-In in Gallus gallus Genome via Microhomology-Mediated End Joining

Precision exogenous gene knock-in is an attractive field for transgenic Gallus gallus (chicken) generation. In this article, we constructed multiple Precise Integration into Target Chromosome (PITCh) plasmid systems mediated by microhomology-mediated end-joining (MMEJ) for large-fragment integration...

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Detalles Bibliográficos
Autores principales: Wang, Lijuan, Sun, Jiaxin, Liu, Zhipeng, Zheng, Qiang, Wang, Guojun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10649300/
https://www.ncbi.nlm.nih.gov/pubmed/37958714
http://dx.doi.org/10.3390/ijms242115731

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10649300/
https://www.ncbi.nlm.nih.gov/pubmed/37958714
http://dx.doi.org/10.3390/ijms242115731

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