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Comparison of Multiple Strategies for Precision Transgene Knock-In in Gallus gallus Genome via Microhomology-Mediated End Joining

Precision exogenous gene knock-in is an attractive field for transgenic Gallus gallus (chicken) generation. In this article, we constructed multiple Precise Integration into Target Chromosome (PITCh) plasmid systems mediated by microhomology-mediated end-joining (MMEJ) for large-fragment integration...

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Detalles Bibliográficos
Autores principales:	Wang, Lijuan, Sun, Jiaxin, Liu, Zhipeng, Zheng, Qiang, Wang, Guojun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10649300/ https://www.ncbi.nlm.nih.gov/pubmed/37958714 http://dx.doi.org/10.3390/ijms242115731

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