Detection of the DNA binding of transcription factors in situ at the single-cell resolution in cultured cells by proximity ligation assay

Transcription factors (TFs) play a pivotal role in gene expression, and their DNA binding is the prerequisite to instigating gene transcription. Here, we present a protocol that exploits the proximity ligation assay technique to measure the DNA-binding activities of TFs in situ at the single-cell re...

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Detalles Bibliográficos
Autores principales: Ram, Babul Moni, Dai, Chengkai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10651771/
https://www.ncbi.nlm.nih.gov/pubmed/37917578
http://dx.doi.org/10.1016/j.xpro.2023.102692

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10651771/
https://www.ncbi.nlm.nih.gov/pubmed/37917578
http://dx.doi.org/10.1016/j.xpro.2023.102692

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