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Detection of the DNA binding of transcription factors in situ at the single-cell resolution in cultured cells by proximity ligation assay
Transcription factors (TFs) play a pivotal role in gene expression, and their DNA binding is the prerequisite to instigating gene transcription. Here, we present a protocol that exploits the proximity ligation assay technique to measure the DNA-binding activities of TFs in situ at the single-cell re...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10651771/ https://www.ncbi.nlm.nih.gov/pubmed/37917578 http://dx.doi.org/10.1016/j.xpro.2023.102692 |