Protocol for identifying physiologically relevant binding proteins of G-protein-coupled receptors

G-protein-coupled receptors (GPCRs) are important therapeutic targets expressed on the cell surface. Here, we present a protocol for identifying physiologically relevant binding proteins of adhesion GPCR GPR110. We describe steps for in-cell chemical crosslinking, immunoprecipitation, and quantitati...

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Detalles Bibliográficos
Autores principales: Huang, Bill X., Kim, Hee-Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10652204/
https://www.ncbi.nlm.nih.gov/pubmed/37925637
http://dx.doi.org/10.1016/j.xpro.2023.102691

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10652204/
https://www.ncbi.nlm.nih.gov/pubmed/37925637
http://dx.doi.org/10.1016/j.xpro.2023.102691

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