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Single-cell isolation from full-thickness human intestinal tissue resections for single-cell RNA sequencing

Single-cell isolation techniques allow the investigation of physical and functional relationships between individual cells within a complex cell population. Here, we present a protocol for single-cell isolation from full-thickness intestinal tissue resections. We describe steps for pre-processing sp...

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Detalles Bibliográficos
Autores principales: West, Gail A., Zhao, Shuai, Nguyen, Quang Tam, Christensen, Stephen M., Gordon, Ilyssa O., Holubar, Stefan D., Kravarik, Kellie M., Fiocchi, Claudio, Mukherjee, Pranab K., Rieder, Florian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10652208/
https://www.ncbi.nlm.nih.gov/pubmed/37925636
http://dx.doi.org/10.1016/j.xpro.2023.102686