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Whole blood RNA extraction efficiency contributes to variability in RNA sequencing data sets

Numerous methodologies are used for blood RNA extraction, and large quantitative differences in recovered RNA content are reported. We evaluated three archived data sets to determine how extraction methodologies might influence mRNA and lncRNA sequencing results. The total quantity of RNA recovered...

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Detalles Bibliográficos
Autores principales: Wilfinger, William W., Eghbalnia, Hamid R., Mackey, Karol, Miller, Robert, Chomczynski, Piotr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10653446/
https://www.ncbi.nlm.nih.gov/pubmed/37972054
http://dx.doi.org/10.1371/journal.pone.0291209