Protocol for arrayed gRNA screening by base editors in mammalian cell lines using lentiviral system

Base editing, a CRISPR-based genome engineering technique, enables precise single-nucleotide modifications while minimizing double-strand breaks. Here, we present a protocol for arrayed mutagenesis using base editors to identify regulatory elements within the gamma-globin locus. We describe steps fo...

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Detalles Bibliográficos
Autores principales: Ravi, Nithin Sam, George, Anila, Mohankumar, Kumarasamypet M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10656259/
https://www.ncbi.nlm.nih.gov/pubmed/37922314
http://dx.doi.org/10.1016/j.xpro.2023.102668

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10656259/
https://www.ncbi.nlm.nih.gov/pubmed/37922314
http://dx.doi.org/10.1016/j.xpro.2023.102668

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