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Protocol for arrayed gRNA screening by base editors in mammalian cell lines using lentiviral system
Base editing, a CRISPR-based genome engineering technique, enables precise single-nucleotide modifications while minimizing double-strand breaks. Here, we present a protocol for arrayed mutagenesis using base editors to identify regulatory elements within the gamma-globin locus. We describe steps fo...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10656259/ https://www.ncbi.nlm.nih.gov/pubmed/37922314 http://dx.doi.org/10.1016/j.xpro.2023.102668 |