Protocol for the saturation and multiplexing of genetic variants using CRISPR-Cas9

Here, we present a multiplexed assay for variant effect protocol to assess the functional impact of all possible genetic variations within a particular genomic region. We describe steps for saturation genome editing by designing and cloning of single-guide RNA (sgRNA). We then detail steps for nucle...

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Detalles Bibliográficos
Autores principales: Sahu, Sounak, Sullivan, Teresa, Southon, Eileen, Caylor, Dylan, Geh, Josephine, Sharan, Shyam K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10658368/
https://www.ncbi.nlm.nih.gov/pubmed/37948185
http://dx.doi.org/10.1016/j.xpro.2023.102702

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10658368/
https://www.ncbi.nlm.nih.gov/pubmed/37948185
http://dx.doi.org/10.1016/j.xpro.2023.102702

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