RNA structure modulates Cas13 activity and enables mismatch detection

The RNA-targeting CRISPR nuclease Cas13 has emerged as a powerful tool for applications ranging from nucleic acid detection to transcriptome engineering and RNA imaging(1–6). Cas13 is activated by the hybridization of a CRISPR RNA (crRNA) to a complementary single-stranded RNA (ssRNA) protospacer in...

Descripción completa

Detalles Bibliográficos
Autores principales: Kimchi, Ofer, Larsen, Benjamin B., Dunkley, Owen R. S., te Velthuis, Aartjan J.W., Myhrvold, Cameron
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10659300/
https://www.ncbi.nlm.nih.gov/pubmed/37987004
http://dx.doi.org/10.1101/2023.10.05.560533

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10659300/
https://www.ncbi.nlm.nih.gov/pubmed/37987004
http://dx.doi.org/10.1101/2023.10.05.560533

Ejemplares similares