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Transcriptome-wide analysis of the function of Ded1 in translation preinitiation complex assembly in a reconstituted in vitro system

We have developed a deep sequencing-based approach, Rec-Seq, that allows simultaneous monitoring of ribosomal 48S pre-initiation complex (PIC) formation on every mRNA in the translatome in an in vitro reconstituted system. Rec-Seq isolates key early steps in translation initiation in the absence of...

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Detalles Bibliográficos
Autores principales: Zhou, Fujun, Bocetti, Julie M., Hou, Meizhen, Qin, Daoming, Hinnebusch, Alan G., Lorsch, Jon R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10659408/
https://www.ncbi.nlm.nih.gov/pubmed/37986768
http://dx.doi.org/10.1101/2023.10.16.562452