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Transcriptome-wide analysis of the function of Ded1 in translation preinitiation complex assembly in a reconstituted in vitro system

We have developed a deep sequencing-based approach, Rec-Seq, that allows simultaneous monitoring of ribosomal 48S pre-initiation complex (PIC) formation on every mRNA in the translatome in an in vitro reconstituted system. Rec-Seq isolates key early steps in translation initiation in the absence of...

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Detalles Bibliográficos
Autores principales:	Zhou, Fujun, Bocetti, Julie M., Hou, Meizhen, Qin, Daoming, Hinnebusch, Alan G., Lorsch, Jon R.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Cold Spring Harbor Laboratory 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10659408/ https://www.ncbi.nlm.nih.gov/pubmed/37986768 http://dx.doi.org/10.1101/2023.10.16.562452

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