TREX2 enables efficient genome disruption mediated by paired CRISPR-Cas9 nickases that generate 3′-overhanging ends

Paired SpCas9 nickases (SpCas9n) are an effective strategy to reduce off-target effect in genome editing. However, this approach is not efficient with 3′-overhanging ends, limiting its applications. In order to expand the utility of paired SpCas9n in genome editing, we tested the effect of the TREX2...

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Detalles Bibliográficos
Autores principales: Wang, Yue, Feng, Yi-Li, Liu, Qian, Xiao, Jing-Jing, Liu, Si-Cheng, Huang, Zhi-Cheng, Xie, An-Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2023
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10661556/
https://www.ncbi.nlm.nih.gov/pubmed/38028195
http://dx.doi.org/10.1016/j.omtn.2023.102072

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10661556/
https://www.ncbi.nlm.nih.gov/pubmed/38028195
http://dx.doi.org/10.1016/j.omtn.2023.102072

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