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Prevalence and molecular characterization of Salmonella isolated from wild birds in fresh produce environments

Wild birds pose a difficult food safety risk to manage because they can avoid traditional wildlife mitigation strategies, such as fences. Birds often use agricultural fields and structures as foraging and nesting areas, which can lead to defecation on crops and subsequent transfer of foodborne patho...

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Autores principales: Smith, Jared C., Varriano, Sofia, Roach, Kerrie, Snipes, Zach, Dawson, Joshua L., Shealy, Justin, Dunn, Laurel L., Snyder, William E., Shariat, Nikki W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10662084/
https://www.ncbi.nlm.nih.gov/pubmed/38029194
http://dx.doi.org/10.3389/fmicb.2023.1272916
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author Smith, Jared C.
Varriano, Sofia
Roach, Kerrie
Snipes, Zach
Dawson, Joshua L.
Shealy, Justin
Dunn, Laurel L.
Snyder, William E.
Shariat, Nikki W.
author_facet Smith, Jared C.
Varriano, Sofia
Roach, Kerrie
Snipes, Zach
Dawson, Joshua L.
Shealy, Justin
Dunn, Laurel L.
Snyder, William E.
Shariat, Nikki W.
author_sort Smith, Jared C.
collection PubMed
description Wild birds pose a difficult food safety risk to manage because they can avoid traditional wildlife mitigation strategies, such as fences. Birds often use agricultural fields and structures as foraging and nesting areas, which can lead to defecation on crops and subsequent transfer of foodborne pathogens. To assess the food safety risk associated with these events, wild bird feces were collected from produce fields across the southeastern United States during the 2021 and 2022 growing seasons. In total 773 fecal samples were collected from 45 farms across Florida, Georgia, South Carolina, and Tennessee, and 2.1% (n = 16) of samples were Salmonella-positive. Importantly, 75% of Salmonella were isolated from moist feces, showing reduced Salmonella viability when feces dry out. 16S microbiome analysis showed that presence of culturable Salmonella in moist feces correlated to a higher proportion of the Enterobacteriaceae family. From the Salmonella-positive samples, 62.5% (10/16) contained multi-serovar Salmonella populations. Overall, 13 serovars were detected, including six most commonly attributed to human illness (Enteriditis, Newport, Typhimurium, Infantis, Saintpaul, and Muenchen). PCR screening identified an additional 59 Salmonella-positive fecal samples, which were distributed across moist (n = 44) and dried feces (n = 15). On-farm point counts and molecular identification from fecal samples identified 57 bird species, including for 10 Salmonella-positive fecal samples. Overall, there was a low prevalence of Salmonella in fecal samples, especially in dried feces, and we found no evidence of Salmonella transmission to proximal foliage or produce. Fecal samples collected in farms close together shared highly related isolates by whole genome sequencing and also had highly similar Salmonella populations with comparable relative frequencies of the same serovars, suggesting the birds acquired Salmonella from a common source.
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spelling pubmed-106620842023-11-07 Prevalence and molecular characterization of Salmonella isolated from wild birds in fresh produce environments Smith, Jared C. Varriano, Sofia Roach, Kerrie Snipes, Zach Dawson, Joshua L. Shealy, Justin Dunn, Laurel L. Snyder, William E. Shariat, Nikki W. Front Microbiol Microbiology Wild birds pose a difficult food safety risk to manage because they can avoid traditional wildlife mitigation strategies, such as fences. Birds often use agricultural fields and structures as foraging and nesting areas, which can lead to defecation on crops and subsequent transfer of foodborne pathogens. To assess the food safety risk associated with these events, wild bird feces were collected from produce fields across the southeastern United States during the 2021 and 2022 growing seasons. In total 773 fecal samples were collected from 45 farms across Florida, Georgia, South Carolina, and Tennessee, and 2.1% (n = 16) of samples were Salmonella-positive. Importantly, 75% of Salmonella were isolated from moist feces, showing reduced Salmonella viability when feces dry out. 16S microbiome analysis showed that presence of culturable Salmonella in moist feces correlated to a higher proportion of the Enterobacteriaceae family. From the Salmonella-positive samples, 62.5% (10/16) contained multi-serovar Salmonella populations. Overall, 13 serovars were detected, including six most commonly attributed to human illness (Enteriditis, Newport, Typhimurium, Infantis, Saintpaul, and Muenchen). PCR screening identified an additional 59 Salmonella-positive fecal samples, which were distributed across moist (n = 44) and dried feces (n = 15). On-farm point counts and molecular identification from fecal samples identified 57 bird species, including for 10 Salmonella-positive fecal samples. Overall, there was a low prevalence of Salmonella in fecal samples, especially in dried feces, and we found no evidence of Salmonella transmission to proximal foliage or produce. Fecal samples collected in farms close together shared highly related isolates by whole genome sequencing and also had highly similar Salmonella populations with comparable relative frequencies of the same serovars, suggesting the birds acquired Salmonella from a common source. Frontiers Media S.A. 2023-11-07 /pmc/articles/PMC10662084/ /pubmed/38029194 http://dx.doi.org/10.3389/fmicb.2023.1272916 Text en Copyright © 2023 Smith, Varriano, Roach, Snipes, Dawson, Shealy, Dunn, Snyder and Shariat. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Smith, Jared C.
Varriano, Sofia
Roach, Kerrie
Snipes, Zach
Dawson, Joshua L.
Shealy, Justin
Dunn, Laurel L.
Snyder, William E.
Shariat, Nikki W.
Prevalence and molecular characterization of Salmonella isolated from wild birds in fresh produce environments
title Prevalence and molecular characterization of Salmonella isolated from wild birds in fresh produce environments
title_full Prevalence and molecular characterization of Salmonella isolated from wild birds in fresh produce environments
title_fullStr Prevalence and molecular characterization of Salmonella isolated from wild birds in fresh produce environments
title_full_unstemmed Prevalence and molecular characterization of Salmonella isolated from wild birds in fresh produce environments
title_short Prevalence and molecular characterization of Salmonella isolated from wild birds in fresh produce environments
title_sort prevalence and molecular characterization of salmonella isolated from wild birds in fresh produce environments
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10662084/
https://www.ncbi.nlm.nih.gov/pubmed/38029194
http://dx.doi.org/10.3389/fmicb.2023.1272916
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