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Adaptation of CUT&RUN for use in African trypanosomes

This Cleavage Under Targets and Release Using Nuclease (CUT&RUN) protocol produces genomic occupancy data for a protein of interest in the protozoan parasite Trypanosoma brucei. The data produced is analyzed in a similar way as that produced by ChIP-seq. While we describe the protocol for parasi...

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Detalles Bibliográficos
Autores principales: Miller, Geneva, Rollosson, Lindsey M., Saada, Carrie, Wade, Serenity J., Schulz, Danae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10662711/
https://www.ncbi.nlm.nih.gov/pubmed/37988382
http://dx.doi.org/10.1371/journal.pone.0292784