Codon optimization of a gene encoding DNA polymerase from Pyrococcus furiosus and its expression in Escherichia coli

BACKGROUND: DNA polymerase is an essential component in PCR assay for DNA synthesis. Improving DNA polymerase with characteristics indispensable for a powerful assay is crucial because it can be used in wide-range applications. Derived from Pyrococcus furiosus, Pfu DNA polymerase (Pfu pol) is one of...

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Detalles Bibliográficos
Autores principales: Nuryana, Isa, Laksmi, Fina Amreta, Dewi, Kartika Sari, Akbar, Faiz Raihan, Nurhayati, Harmoko, Rikno
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10663413/
https://www.ncbi.nlm.nih.gov/pubmed/37987973
http://dx.doi.org/10.1186/s43141-023-00605-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10663413/
https://www.ncbi.nlm.nih.gov/pubmed/37987973
http://dx.doi.org/10.1186/s43141-023-00605-7

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