A quantitative sequencing method using synthetic internal standards including functional and phylogenetic marker genes

The method of spiking synthetic internal standard genes (ISGs) to samples for amplicon sequencing, generating sequences and converting absolute gene numbers from read counts has been used only for phylogenetic markers and has not been applied to functional markers. In this study, we developed ISGs,...

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Detalles Bibliográficos
Autores principales: Koike, Kazuyoshi, Honda, Ryo, Aoki, Masataka, Yamamoto‐Ikemoto, Ryoko, Syutsubo, Kazuaki, Matsuura, Norihisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2023
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10667660/
https://www.ncbi.nlm.nih.gov/pubmed/37465846
http://dx.doi.org/10.1111/1758-2229.13189

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10667660/
https://www.ncbi.nlm.nih.gov/pubmed/37465846
http://dx.doi.org/10.1111/1758-2229.13189

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