Pyramiding stacking of multigenes (PSM): a simple, flexible and efficient multigene stacking system based on Gibson assembly and gateway cloning

Genetic engineering of complex metabolic pathways and multiple traits often requires the introduction of multiple genes. The construction of plasmids carrying multiple DNA fragments plays a vital role in these processes. In this study, the Gibson assembly and Gateway cloning combined Pyramiding Stac...

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Detalles Bibliográficos
Autores principales: Zeng, Dongdong, Jing, Cuiyuan, Tang, Lin, He, Peng, Zhang, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Bioengineering and Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10668122/
https://www.ncbi.nlm.nih.gov/pubmed/38026899
http://dx.doi.org/10.3389/fbioe.2023.1263715

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10668122/
https://www.ncbi.nlm.nih.gov/pubmed/38026899
http://dx.doi.org/10.3389/fbioe.2023.1263715

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