Cargando…
Pyramiding stacking of multigenes (PSM): a simple, flexible and efficient multigene stacking system based on Gibson assembly and gateway cloning
Genetic engineering of complex metabolic pathways and multiple traits often requires the introduction of multiple genes. The construction of plasmids carrying multiple DNA fragments plays a vital role in these processes. In this study, the Gibson assembly and Gateway cloning combined Pyramiding Stac...
Autores principales: | Zeng, Dongdong, Jing, Cuiyuan, Tang, Lin, He, Peng, Zhang, Jie |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10668122/ https://www.ncbi.nlm.nih.gov/pubmed/38026899 http://dx.doi.org/10.3389/fbioe.2023.1263715 |
Ejemplares similares
-
YALIcloneNHEJ: An Efficient Modular Cloning Toolkit for NHEJ Integration of Multigene Pathway and Terpenoid Production in Yarrowia lipolytica
por: Li, Ya-Wen, et al.
Publicado: (2022) -
Plant Metabolic Engineering by Multigene Stacking: Synthesis of Diverse Mogrosides
por: Liao, Jingjing, et al.
Publicado: (2022) -
Synthesis of Crocin I and Crocin II by Multigene Stacking in Nicotiana benthamiana
por: Xie, Lei, et al.
Publicado: (2023) -
A Modular Cloning System for Standardized Assembly of Multigene Constructs
por: Weber, Ernst, et al.
Publicado: (2011) -
Recombinase‐mediated integration of a multigene cassette in rice leads to stable expression and inheritance of the stacked locus
por: Pathak, Bhuvan, et al.
Publicado: (2020)