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CRISPR/Cas12a Collateral Cleavage Activity for Sensitive 3′–5′ Exonuclease Assay

This study presents a technique for detecting 3′–5′ exonuclease activity through the use of CRISPR/Cas12a. These enzymes, including 3′–5′ exonuclease (Exo III), perform crucial roles in various cellular processes and are associated with life expectancy. However, imbalances in their expression can in...

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Detalles Bibliográficos
Autores principales: Jeung, Jae Hoon, Han, Hyogu, Lee, Chang Yeol, Ahn, Jun Ki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10669037/
https://www.ncbi.nlm.nih.gov/pubmed/37998138
http://dx.doi.org/10.3390/bios13110963