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Transient Expression in HEK-293 Cells in Suspension Culture as a Rapid and Powerful Tool: SARS-CoV-2 N and Chimeric SARS-CoV-2N-CD154 Proteins as a Case Study

In a previous work, we proposed a vaccine chimeric antigen based on the fusion of the SARS-CoV-2 N protein to the extracellular domain of the human CD40 ligand (CD154). This vaccine antigen was named N-CD protein and its expression was carried out in HEK-293 stably transfected cells, grown in adhere...

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Detalles Bibliográficos
Autores principales: Lao, Thailin, Farnos, Omar, Bueno, Alexi, Alvarez, Anays, Rodríguez, Elsa, Palacios, Julio, de la Luz, Kathya Rashida, Kamen, Amine, Carpio, Yamila, Estrada, Mario Pablo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10669214/
https://www.ncbi.nlm.nih.gov/pubmed/38002050
http://dx.doi.org/10.3390/biomedicines11113050