Cargando…
Transient Expression in HEK-293 Cells in Suspension Culture as a Rapid and Powerful Tool: SARS-CoV-2 N and Chimeric SARS-CoV-2N-CD154 Proteins as a Case Study
In a previous work, we proposed a vaccine chimeric antigen based on the fusion of the SARS-CoV-2 N protein to the extracellular domain of the human CD40 ligand (CD154). This vaccine antigen was named N-CD protein and its expression was carried out in HEK-293 stably transfected cells, grown in adhere...
Autores principales: | Lao, Thailin, Farnos, Omar, Bueno, Alexi, Alvarez, Anays, Rodríguez, Elsa, Palacios, Julio, de la Luz, Kathya Rashida, Kamen, Amine, Carpio, Yamila, Estrada, Mario Pablo |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10669214/ https://www.ncbi.nlm.nih.gov/pubmed/38002050 http://dx.doi.org/10.3390/biomedicines11113050 |
Ejemplares similares
-
Production and characterization of a chimeric antigen, based on nucleocapsid of SARS-CoV-2 fused to the extracellular domain of human CD154 in HEK-293 cells as a vaccine candidate against COVID-19
por: Lao, Thailin, et al.
Publicado: (2023) -
Silencing of SARS-CoV spike gene by small interfering RNA in HEK 293T cells
por: Qin, Zhao-ling, et al.
Publicado: (2004) -
Chimeric Antigen by the Fusion of SARS-CoV-2 Receptor Binding Domain with the Extracellular Domain of Human CD154: A Promising Improved Vaccine Candidate
por: Ávalos, Ileanet, et al.
Publicado: (2022) -
Effect of insilico predicted and designed potential siRNAs on inhibition of SARS-CoV-2 in HEK-293 cells
por: Sohrab, Sayed Sartaj, et al.
Publicado: (2022) -
Designing and evaluation of MERS-CoV siRNAs in HEK-293 cell line
por: Sohrab, Sayed Sartaj, et al.
Publicado: (2021)