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Sweet-Tasting Natural Proteins Brazzein and Monellin: Safe Sugar Substitutes for the Food Industry

This article presents the results of a comprehensive toxicity assessment of brazzein and monellin, yeast-produced recombinant sweet-tasting proteins. Excessive sugar consumption is one of the leading dietary and nutritional problems in the world, resulting in health complications such as obesity, hi...

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Autores principales: Novik, Tamara S., Koveshnikova, Elena I., Kotlobay, Anatoly A., Sycheva, Lyudmila P., Kurochkina, Karine G., Averina, Olga A., Belopolskaya, Maria V., Sergiev, Petr V., Dontsova, Olga A., Lazarev, Vassili N., Maev, Igor V., Kostyaeva, Margarita G., Eremeev, Artem V., Chukina, Svetlana I., Lagarkova, Maria A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10670179/
https://www.ncbi.nlm.nih.gov/pubmed/38002123
http://dx.doi.org/10.3390/foods12224065
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author Novik, Tamara S.
Koveshnikova, Elena I.
Kotlobay, Anatoly A.
Sycheva, Lyudmila P.
Kurochkina, Karine G.
Averina, Olga A.
Belopolskaya, Maria V.
Sergiev, Petr V.
Dontsova, Olga A.
Lazarev, Vassili N.
Maev, Igor V.
Kostyaeva, Margarita G.
Eremeev, Artem V.
Chukina, Svetlana I.
Lagarkova, Maria A.
author_facet Novik, Tamara S.
Koveshnikova, Elena I.
Kotlobay, Anatoly A.
Sycheva, Lyudmila P.
Kurochkina, Karine G.
Averina, Olga A.
Belopolskaya, Maria V.
Sergiev, Petr V.
Dontsova, Olga A.
Lazarev, Vassili N.
Maev, Igor V.
Kostyaeva, Margarita G.
Eremeev, Artem V.
Chukina, Svetlana I.
Lagarkova, Maria A.
author_sort Novik, Tamara S.
collection PubMed
description This article presents the results of a comprehensive toxicity assessment of brazzein and monellin, yeast-produced recombinant sweet-tasting proteins. Excessive sugar consumption is one of the leading dietary and nutritional problems in the world, resulting in health complications such as obesity, high blood pressure, and cardiovascular disease. Although artificial small-molecule sweeteners widely replace sugar in food, their safety and long-term health effects remain debatable. Many sweet-tasting proteins, including thaumatin, miraculin, pentadin, curculin, mabinlin, brazzein, and monellin have been found in tropical plants. These proteins, such as brazzein and monellin, are thousands-fold sweeter than sucrose. Multiple reports have presented preparations of recombinant sweet-tasting proteins. A thorough and comprehensive assessment of their toxicity and safety is necessary to introduce and apply sweet-tasting proteins in the food industry. We experimentally assessed acute, subchronic, and chronic toxicity effects, as well as allergenic and mutagenic properties of recombinant brazzein and monellin. Our study was performed on three mammalian species (mice, rats, and guinea pigs). Assessment of animals’ physiological, biochemical, hematological, morphological, and behavioral indices allows us to assert that monellin and brazzein are safe and nontoxic for the mammalian organism, which opens vast opportunities for their application in the food industry as sugar alternatives.
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spelling pubmed-106701792023-11-08 Sweet-Tasting Natural Proteins Brazzein and Monellin: Safe Sugar Substitutes for the Food Industry Novik, Tamara S. Koveshnikova, Elena I. Kotlobay, Anatoly A. Sycheva, Lyudmila P. Kurochkina, Karine G. Averina, Olga A. Belopolskaya, Maria V. Sergiev, Petr V. Dontsova, Olga A. Lazarev, Vassili N. Maev, Igor V. Kostyaeva, Margarita G. Eremeev, Artem V. Chukina, Svetlana I. Lagarkova, Maria A. Foods Article This article presents the results of a comprehensive toxicity assessment of brazzein and monellin, yeast-produced recombinant sweet-tasting proteins. Excessive sugar consumption is one of the leading dietary and nutritional problems in the world, resulting in health complications such as obesity, high blood pressure, and cardiovascular disease. Although artificial small-molecule sweeteners widely replace sugar in food, their safety and long-term health effects remain debatable. Many sweet-tasting proteins, including thaumatin, miraculin, pentadin, curculin, mabinlin, brazzein, and monellin have been found in tropical plants. These proteins, such as brazzein and monellin, are thousands-fold sweeter than sucrose. Multiple reports have presented preparations of recombinant sweet-tasting proteins. A thorough and comprehensive assessment of their toxicity and safety is necessary to introduce and apply sweet-tasting proteins in the food industry. We experimentally assessed acute, subchronic, and chronic toxicity effects, as well as allergenic and mutagenic properties of recombinant brazzein and monellin. Our study was performed on three mammalian species (mice, rats, and guinea pigs). Assessment of animals’ physiological, biochemical, hematological, morphological, and behavioral indices allows us to assert that monellin and brazzein are safe and nontoxic for the mammalian organism, which opens vast opportunities for their application in the food industry as sugar alternatives. MDPI 2023-11-08 /pmc/articles/PMC10670179/ /pubmed/38002123 http://dx.doi.org/10.3390/foods12224065 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Novik, Tamara S.
Koveshnikova, Elena I.
Kotlobay, Anatoly A.
Sycheva, Lyudmila P.
Kurochkina, Karine G.
Averina, Olga A.
Belopolskaya, Maria V.
Sergiev, Petr V.
Dontsova, Olga A.
Lazarev, Vassili N.
Maev, Igor V.
Kostyaeva, Margarita G.
Eremeev, Artem V.
Chukina, Svetlana I.
Lagarkova, Maria A.
Sweet-Tasting Natural Proteins Brazzein and Monellin: Safe Sugar Substitutes for the Food Industry
title Sweet-Tasting Natural Proteins Brazzein and Monellin: Safe Sugar Substitutes for the Food Industry
title_full Sweet-Tasting Natural Proteins Brazzein and Monellin: Safe Sugar Substitutes for the Food Industry
title_fullStr Sweet-Tasting Natural Proteins Brazzein and Monellin: Safe Sugar Substitutes for the Food Industry
title_full_unstemmed Sweet-Tasting Natural Proteins Brazzein and Monellin: Safe Sugar Substitutes for the Food Industry
title_short Sweet-Tasting Natural Proteins Brazzein and Monellin: Safe Sugar Substitutes for the Food Industry
title_sort sweet-tasting natural proteins brazzein and monellin: safe sugar substitutes for the food industry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10670179/
https://www.ncbi.nlm.nih.gov/pubmed/38002123
http://dx.doi.org/10.3390/foods12224065
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